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Determination of elastin-antielastin circulating immune complexes in serum of normal subjects and atherosclerotic patients.

Authors
  • Baydanoff, S
  • Nicoloff, A G
Type
Published Article
Journal
Cor et vasa
Publication Date
Jan 01, 1991
Volume
33
Issue
4
Pages
282–287
Identifiers
PMID: 1743021
Source
Medline
License
Unknown

Abstract

Healthy subjects of different ages and a group of atherosclerotic patients were tested for the presence of elastin-antielastin circulating immune complexes (CIC) in their sera. For the purpose the authors used a method based on sequential precipitation with rising concentrations of polyethylene glycol (PEG), dissociation of the precipitated CIC and further analysis of the resuspended precipitates for the presence of elastin derived peptides by an enzyme-linked immunosorbent assay (ELISA). Elastin-antielastin CIC were detected only in the serum of atherosclerotic patients and that of healthy subjects over 60. The elastin-antielastin CIC obtained from the serum of atherosclerotic patients were precipitated by higher PEG concentrations mainly and had a higher elastin content in comparison with those isolated from the serum of healthy persons over 60.

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