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[Determination of creatinine in urine by high performance liquid chromatography (HPLC) (author's transl)].

Authors
  • Hausen, A
  • Fuchs, D
  • Wachter, H
Type
Published Article
Journal
Journal of clinical chemistry and clinical biochemistry. Zeitschrift für klinische Chemie und klinische Biochemie
Publication Date
Jun 01, 1981
Volume
19
Issue
6
Pages
375–378
Identifiers
PMID: 7276834
Source
Medline
License
Unknown

Abstract

A simple, specific method is described for the determination of creatinine in urine by HPLC. A C18-reversed-phase column is used for the separation. Detection is with a spectral UV-absorption photometer at 235 nm. The results for the precision and the accuracy meet the guidelines of the statistical quality control. Comparative investigations using the method by Lim et al. ((1978), J. Chromatogr. 67, 182-187) matched well with a coefficient of reference of 0.994. The specificity of the method was confirmed by the UV-absorption spectrum of creatinine isolated by HPLC. In addition, 28 urinary constituents were tested for possible interference.

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