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Determination of betamethasone in human plasma by liquid chromatography with tandem mass.

Authors
  • Qu, Ting-Ting
  • Zhang, Rui
  • Wang, Ben-Jie
  • Liu, Xiao-Yan
  • Yuan, Gui-Yan
  • Guo, Rui-Chen
Type
Published Article
Journal
Yao xue xue bao = Acta pharmaceutica Sinica
Publication Date
Apr 01, 2008
Volume
43
Issue
4
Pages
402–407
Identifiers
PMID: 18664204
Source
Medline
License
Unknown

Abstract

A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of betamethasone in human plasma. The analyte was isocratically eluted on a Venusil XBP C8 column (200 mm x 3.9 mm ID, 5 microm) with methanol-water mol x L(-1) ammonium formate) (80:20) at a flow rate of 0.4 mL x min(-1), and detected (containing 5 mmol x L(-1) ammonium formate) (80:20) at a flow rate of 0.4 mL x min(-1), and detected with a triple quad LC-MS/MS using ESI with positive ionization. Ions monitored in the multiple reaction monitoring (MRM) mode were m/z 393.3-->355.2 for betamethasone and m/z 361.3-->343.2 for prednisolone (IS). Betamethasone was extracted from 0.5 mL human plasma with ethyl acetate. The average recovery is 88.24% and the low limit of quantitation (LLOQ) was 0.5 ng x mL(-1). The 3-day validation study demonstrated excellent precision and accuracy across the calibration range of 0.5-80.0 ng x mL(-1). The method was successfully applied to the pharmacokinetic study of compound betamethason injection in healthy Chinese volunteers.

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