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Determination of aqueous two-phase system phase-forming components in the presence of bovine serum albumin

Authors
  • Glyk, Anna
  • Scheper, Thomas
  • Beutel, Sascha
Type
Published Article
Journal
Analytical Biochemistry
Publication Date
Jan 01, 2014
Accepted Date
Mar 13, 2014
Identifiers
DOI: 10.1016/j.ab.2014.03.009
Source
Elsevier
Keywords
License
Unknown

Abstract

In the present work, the quantification of different poly(ethylene glycol) (PEG) + potassium phosphate / sodium citrate aqueous two-phase system (ATPS) phase-forming components was investigated by using conductivity and refractive index measurements. For this purpose, refractive index and conductivity calibration curves were obtained for ATPS at different pH values in the presence of different bovine serum albumin (BSA) concentrations. While BSA had no effect on the conductivity, it had a considerable effect on the refractive index. Finally, a convenient dilution of the samples prior to the ATPS constituent determination is needed to ensure no significant influence from BSA.

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