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Determination of amyloid beta protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA.

Authors
  • Kitaguchi, N
  • Tokushima, Y
  • Oishi, K
  • Takahashi, Y
  • Shiojiri, S
  • Nakamura, S
  • Tanaka, S
  • Kodaira, R
  • Ito, H
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Feb 14, 1990
Volume
166
Issue
3
Pages
1453–1459
Identifiers
PMID: 2106318
Source
Medline
License
Unknown

Abstract

beta-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.

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