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Determination of 2-keto- l-gulonic and other ketoaldonic and aldonic acids produced by ketogenic bacterial fermentation

Authors
  • Lazarus, Robert A.
  • Seymour, Jana L.
Type
Published Article
Journal
Analytical Biochemistry
Publisher
Elsevier BV
Publication Date
Jan 01, 1986
Volume
157
Issue
2
Pages
360–366
Identifiers
DOI: 10.1016/0003-2697(86)90638-X
Source
Elsevier
Keywords
License
Unknown

Abstract

The quantitative analysis of 2-keto- l-gulonic acid (2-KLG) produced by microbial fermentation is described. 2-KLG is separated from other aldonic and ketoaldonic acids by high-performance liquid chromatography on an Aminex anion exchange column with ammonium formate or potassium phosphate as the eluant. This is a rapid and simple method for routine analysis of a large number of samples generated by fermentation studies. Gas chromatography—mass spectrometry permits the qualitative and quantitative analysis of nanogram levels of 2-keto- l-gulonate in complex media and provides confirmation of the HPLC results. The methodologies presented are useful for the analysis of a number of aldonic and ketoaldonic acids.

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