Affordable Access

Detection of water-borne E. coli O157 using the integrating waveguide biosensor.

Authors
  • Zhu, Peixuan
  • Shelton, Daniel R
  • Karns, Jeffrey S
  • Sundaram, Appavu
  • Li, Shuhong
  • Amstutz, Pete
  • Tang, Cha-Mei
Type
Published Article
Journal
Biosensors and Bioelectronics
Publisher
Elsevier
Publication Date
Oct 15, 2005
Volume
21
Issue
4
Pages
678–683
Identifiers
PMID: 16202883
Source
Medline
License
Unknown

Abstract

Escherichia coli O157:H7, the most common serotype of enterohemorrhagic E. coli (EHEC), is responsible for numerous food-borne and water-borne infections worldwide. An integrating waveguide biosensor is described for the detection of water-borne E. coli O157, based on a fluorescent sandwich immunoassay performed inside a glass capillary waveguide. The genomic DNA of captured E. coli O157 cells was extracted and quantitative real-time PCR subsequently performed to assess biosensor-capture efficiency. In vitro microbial growth in capillary waveguide is also documented. The biosensor allows for quantitative detection of as few as 10 cells per capillary (0.075 ml volume) and can be used in conjunction with cell amplification, PCR and microarray technologies to positively identify a pathogen.

Report this publication

Statistics

Seen <100 times