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[Detection of tissue factor-positive microparticles and its clinical significance in the haemostatic disorder].

Authors
  • Zhou, Li-Li
  • Han, Yue
  • Zhu, Qian
  • Hu, Lu-Ping
  • Zhao, Shi-Xiang
  • Zhu, Ming-Qing
  • Dai, Lan
  • Shen, Wen-Hong
  • Chen, Li
  • Wu, De-Pei
Type
Published Article
Journal
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology
Publication Date
Aug 01, 2012
Volume
20
Issue
4
Pages
937–940
Identifiers
PMID: 22931659
Source
Medline
License
Unknown

Abstract

Objective of this study was to detect the level of tissue factor-positive microparticles (TF(+)MP) by flow cytometry (FCM) and to analyze its clinical significance in the haemostatic disorder. TF(+) MP was detected by FCM using antibody CD142-PE in 25 cases of acute promyelocytic leukemia (APL), 20 cases of hemostatic diseases and 20 healthy adults as controls. The differences of TF(+) MP between various groups were determined. The results showed that the level of TF(+) MP in the patients with thrombotic complications was significantly higher than that in the healthy adults (P < 0.05). The TF(+) MP level was higher in the patient with APL than that in the healthy adults, especially in course before therapy (P < 0.01), but the difference was not statistically significant in the patient with APL after therapy and the healthy adults. Among these patient with APL, the level of TF(+) MP in the 18 patients who complicated with disseminated intravascular coagulation (DIC) was also higher than that in the healthy adults (P < 0.05), but the level of TF(+) MP in the other 7 patients who did not complicate with DIC was similar before and after treatment. It is concluded that the method of TF(+) MP detection by FCM is feasible and simple, it is useful for the diagnosis of thrombotic disorder, and helps evaluation for the prognosis of APL patient.

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