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Detection of RNA on northern blots by negative staining with aurintricarboxylic acid.

Authors
  • Oberbäumer, I
  • Speth, C
Type
Published Article
Journal
Analytical biochemistry
Publication Date
Feb 15, 1990
Volume
185
Issue
1
Pages
77–79
Identifiers
PMID: 1693051
Source
Medline
License
Unknown

Abstract

Aurintricarboxylic acid (ATA) is a well-known inhibitor of RNA and DNA modifying enzymes and was suggested as a potent RNase inhibitor for preparation of RNA (Hallick et al., 1977, Nucleic Acids Res. 4, 3055-3064). We show that ATA is a very useful stain for detecting RNA on Northern blots and slot blots although it did not fully protect purified RNA in concentrated solution against RNase A.

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