Affordable Access

Detection and rapid differentiation of human enteroviruses in water sources by restriction enzyme analysis.

Authors
  • Vivier, J C
  • Clay, C G
  • Grabow, W O
Type
Published Article
Journal
Water science and technology : a journal of the International Association on Water Pollution Research
Publication Date
Jan 01, 2001
Volume
43
Issue
12
Pages
209–212
Identifiers
PMID: 11464758
Source
Medline
License
Unknown

Abstract

The objective of this study was to assess the application and efficiency of molecular techniques for the detection and serotyping of enteroviruses from environmental water samples. Samples of water were collected at regular intervals upstream and downstream of an informal settlement. Techniques for the detection of enteroviruses included a reverse transcription polymerase chain reaction (RT-PCR), nested PCR (n-PCR) and Sabin-specific triplex PCR. A specific 297 bp fragment was amplified by the n-PCR and subjected to restriction enzyme (RE) analysis to differentiate between various serotypes of prototypical enteroviruses. Enteroviruses that gave inconclusive restriction patterns were typed by partial sequencing of the VP1 region. Results indicated a high incidence of enteroviruses, predominantly coxsackie B viruses. The results on polioviruses, as well as other enteroviruses, contributed valuable information on enteroviruses circulating in the community. The molecular approach described here proved suitable for the rapid, sensitive, specific and cost effective, simultaneous detection and typing of enteroviruses in water.

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