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Detection and quantitative determination of 3-nitropropionic acid in bovine urine.

Authors
  • Majak, W1
  • McDiarmid, R E
  • 1 Research Station, Agriculture Canada, Kamloops, BC. , (Canada)
Type
Published Article
Journal
Toxicology Letters
Publisher
Elsevier
Publication Date
Feb 01, 1990
Volume
50
Issue
2-3
Pages
213–220
Identifiers
PMID: 2309240
Source
Medline
License
Unknown

Abstract

The separation and determination of 3-nitropropionic acid (NPA) in bovine urine by reverse-phase HPLC is described. The method utilizes a rapid ethyl acetate extraction and this clean-up step also improves the chromatographic resolution of NPA in bovine plasma. A simple colorimetric method is also developed for the rapid detection and estimation of NPA in urine and the data indicate that it is in good agreement with the HPLC procedure.

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