Detection and quantitative determination of 3-nitropropionic acid in bovine urine.
Research Station, Agriculture Canada, Kamloops, BC.
- Published Article
- Publication Date
Feb 01, 1990
The separation and determination of 3-nitropropionic acid (NPA) in bovine urine by reverse-phase HPLC is described. The method utilizes a rapid ethyl acetate extraction and this clean-up step also improves the chromatographic resolution of NPA in bovine plasma. A simple colorimetric method is also developed for the rapid detection and estimation of NPA in urine and the data indicate that it is in good agreement with the HPLC procedure.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/2309240