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Detection of pullulanase in polyacrylamide gels using pullulan-reactive red agar plates.

Authors
Type
Published Article
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
160
Issue
2
Pages
480–482
Identifiers
PMID: 3555152
Source
Medline

Abstract

After electrophoresis, active pullulanase bands in acrylamide gels have been detected by overlaying and then incubating the gel on a replica gel containing 2.5% pullulan-reactive red conjugate and 2.1% agar. The enzyme activity is revealed as a clear band against a red background on the replica gel. The sensitivity of the replica plate is such that 0.0012 unit of Klebsiella aerogenes pullulanase can be detected easily. This procedure is effective in enzyme screening to distinguish pullulanase from other carbohydrases.

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