Detection of pullulanase in polyacrylamide gels using pullulan-reactive red agar plates.
- Published Article
- Publication Date
Feb 01, 1987
After electrophoresis, active pullulanase bands in acrylamide gels have been detected by overlaying and then incubating the gel on a replica gel containing 2.5% pullulan-reactive red conjugate and 2.1% agar. The enzyme activity is revealed as a clear band against a red background on the replica gel. The sensitivity of the replica plate is such that 0.0012 unit of Klebsiella aerogenes pullulanase can be detected easily. This procedure is effective in enzyme screening to distinguish pullulanase from other carbohydrases.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/3555152