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The detection and prevalence of Clostridium botulinum in pig intestinal samples.

Authors
  • Myllykoski, Jan
  • Nevas, Mari
  • Lindström, Miia
  • Korkeala, Hannu
Type
Published Article
Journal
International Journal of Food Microbiology
Publisher
Elsevier
Publication Date
Jul 15, 2006
Volume
110
Issue
2
Pages
172–177
Identifiers
PMID: 16806550
Source
Medline
License
Unknown

Abstract

To determine the prevalence of Clostridium botulinum types A, B, E, and/or F in pig intestinal samples, different PCR-based methods were compared using artificially contaminated faeces. The methods included a multiplex PCR targeted to botulinum neurotoxin genes A, B, E, and F or a nested PCR targeted to toxin genes B, E, and F, combined with different pre-enrichment protocols and forms of templates. A method including the two-step enrichment followed by DNA extraction and multiplex PCR yielded the highest number of positives. This assay protocol was employed to investigate 100 pig intestinal samples. The sample materials studied included colon wall, intestinal content, and mucus peeled from the colon wall. Three pigs (3%) were positive for C. botulinum type B, and no other toxinotypes were detected in any sample. The number of positive samples was higher when colon wall or peeled mucus was analyzed compared to the intestinal content. C. botulinum was isolated from two PCR-positive samples and confirmed to be type B by PCR. Both isolates were shown to be proteolytic and thus to represent C. botulinum group I.

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