Affordable Access

Detection of point mutations in the p53 gene: comparison of single-strand conformation polymorphism, constant denaturant gel electrophoresis, and hydroxylamine and osmium tetroxide techniques.

Authors
  • Condie, A
  • Eeles, R
  • Borresen, A L
  • Coles, C
  • Cooper, C
  • Prosser, J
Type
Published Article
Journal
Human mutation
Publication Date
Jan 01, 1993
Volume
2
Issue
1
Pages
58–66
Identifiers
PMID: 8477265
Source
Medline
License
Unknown

Abstract

A comparison was made between the 3 most commonly used techniques for the detection of point mutations: single-strand conformation polymorphism (SSCP), constant denaturant gel electrophoresis (CDGE), and hydroxylamine and osmium tetroxide used in amplification mismatch cleavage analysis (HOT). Using human DNA samples containing known mutations in the p53 gene, SSCP detected 90% of mutations (18/20), CDGE detected 88% (15/17) pre-decoding of the samples but 100% when the mutations were known and the CDGE conditions optimized, and the HOT technique was 100% accurate, although 1 mutation was missed through careless examination of the gel. The positive and negative aspects of each of the techniques are considered and suggestions are made regarding the particular situations in which each of them is most useful.

Report this publication

Statistics

Seen <100 times