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Detection and phylogeny of Staphylococcus aureus sequence type 398 in Taiwan

Authors
  • Huang, Yhu-Chering1, 2, 3
  • Chen, Chih-Jung1, 2
  • 1 Chang Gung Memorial Hospital, Taoyuan, Taiwan , Taoyuan (Taiwan)
  • 2 Chang Gung University College of Medicine, Taoyuan, Taiwan , Taoyuan (Taiwan)
  • 3 Chang Gung Memorial Hospital, No. 5, Fu-Shin Street, Gueishan, Taoyuan, 333, Taiwan , Taoyuan (Taiwan)
Type
Published Article
Journal
Journal of Biomedical Science
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jan 03, 2020
Volume
27
Issue
1
Identifiers
DOI: 10.1186/s12929-019-0608-8
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) ST398 is a livestock associated-bacterium that is most prevalent in Europe. Human-adapted MRSA ST398 was recently reported from China, but there is no data available yet for Taiwan.MethodsTo identify S. aureus ST398 isolates, we examined 6413 S. aureus isolates (5632 MRSA and 781 susceptible strains) that were collected in Taiwan between 1995 and 2017. If isolates could not be typed by pulsed-field gel electrophoresis upon Sma I digestion, we performed further characterization and complete genome sequencing.ResultsWe identified 18 ST398 S. aureus isolates from 16 subjects (0.28%), including 6 sensitive and 12 resistant strains. Of these, 14 were colonizing isolates, 3 were clinical (infecting) isolates and one isolate was from a pork specimen. All 3 infecting isolates were MSSA strains identified in 2015 from two children with recurrent otitis media or sinusitis. The other 3 MSSA isolates were identified from workers handling pork (2) or pork meat (1) in 2015. The first 5 MRSA colonizing isolates were identified from residents in two nursing homes in 2012. Six MRSA isolates were identified from residents and foreign employees at a nursing home in 2016 and one MRSA from a foreign worker in 2017. Phylogenetic analysis of genome sequences indicated that all 12 local ST398 MRSA strains cluster together, human-adapted and phylogenetically related to a human MRSA strain identified in China in 2002. Two local MSSA isolates could be linked to isolates from livestock. The toxin profiles were similar for the MRSA and MSSA isolates.ConclusionsOur results demonstrate that S. aureus ST398 was present in Taiwan in 2012 and potentially earlier. Although some isolates could be linked to livestock, most ST398 S. aureus isolates identified in Taiwan, particularly MRSA, represent human-adapted strains. Local transmission of human-adapted MRSA ST398 strains has occurred in nursing homes in Taiwan, possibly after import from China. Further surveillance is needed.

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