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Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.

Authors
  • Corman, V M
  • Eckerle, I
  • Bleicker, T
  • Zaki, A
  • Landt, O
  • Eschbach-Bludau, M
  • van Boheemen, S
  • Gopal, R
  • Ballhause, M
  • Bestebroer, T M
  • Muth, D
  • Müller, M A
  • Drexler, J F
  • Zambon, M
  • Osterhaus, A D
  • Fouchier, R M
  • Drosten, C
Type
Published Article
Journal
Eurosurveillance
Publisher
European Centre for Disease Control and Prevention (ECDC)
Publication Date
Jan 01, 2012
Volume
17
Issue
39
Identifiers
PMID: 23041020
Source
Medline
License
Unknown

Abstract

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

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