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Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.

Authors
Type
Published Article
Journal
Eurosurveillance
1560-7917
Publisher
European Centre for Disease Control and Prevention (ECDC)
Publication Date
Volume
17
Issue
39
Identifiers
PMID: 23041020
Source
Medline
License
Unknown

Abstract

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

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