Affordable Access

Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction.

Authors
  • Chaharaein, B
  • Omar, A R
  • Aini, I
  • Yusoff, K
  • Hassan, S S
Type
Published Article
Journal
Microbiological Research
Publisher
Elsevier
Publication Date
Jan 01, 2009
Volume
164
Issue
2
Pages
174–179
Identifiers
PMID: 17336046
Source
Medline
License
Unknown

Abstract

Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR-enzyme-linked immunosorbent assay (ELISA) and conventional RT-PCR. While the sensitivity of the multiplex RT-PCR is as sensitive as the conventional RT-PCR, it is 10 times less sensitive than RT-PCR-ELISA. The multiplex RT-PCR is also as sensitive as the virus isolation method in detecting H9N2 from tracheal samples collected at day 3 and 5 post inoculation. Hence, the developed multiplex RT-PCR assay is a rapid, sensitive and specific assay for detecting of AIV subtypes.

Report this publication

Statistics

Seen <100 times