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Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA

Authors
  • Wang, Zhen1
  • Maluenda, Jérôme1
  • Giraut, Laurène1
  • Vieille, Thibault1
  • Lefevre, Andréas1
  • Salthouse, David1
  • Radou, Gaël1
  • Moulinas, Rémi1
  • Astete, Sandra1
  • D’Avezac, Pol1
  • Smith, Geoff1
  • André, Charles1
  • Allemand, Jean-François2, 3
  • Bensimon, David2, 3, 4
  • Croquette, Vincent2, 3, 5
  • Ouellet, Jimmy1
  • Hamilton, Gordon1
  • 1 Depixus SAS, 3/5 Impasse Reille, Paris, 75014, France , Paris (France)
  • 2 Laboratoire de physique de L’École normale supérieure de Paris, CNRS, ENS, Université PSL, Sorbonne Université, Université de Paris, Paris, 75005, France , Paris (France)
  • 3 École normale supérieure, CNRS, INSERM, PSL Research University, Paris, 75005, France , Paris (France)
  • 4 UCLA, 607 Charles E Young Drive East, Los Angeles, 90095, USA , Los Angeles (United States)
  • 5 PSL University, 10 rue Vauquelin, Paris, 75005, France , Paris (France)
Type
Published Article
Journal
Communications Biology
Publisher
Nature Publishing Group UK
Publication Date
Jan 29, 2021
Volume
4
Issue
1
Identifiers
DOI: 10.1038/s42003-021-01648-7
Source
Springer Nature
License
Green

Abstract

Wang et al. show how genetic sequence and base modifications can be detected simultaneously on single molecules of both DNA and RNA using magnetic tweezers. They also demonstrate an amplification-free CRISPR/Cas9-based strategy for isolating target regions from native DNA and apply this approach to the isolation of targets from E. coli and human genomic DNA.

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