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Detection of four adenovirus serotypes within water-isolated strains of Acanthamoeba in the Canary Islands, Spain.

Authors
  • Lorenzo-Morales, Jacob
  • Coronado-Alvarez, Nieves
  • Martínez-Carretero, Enrique
  • Maciver, Sutherland K
  • Valladares, Basilio
Type
Published Article
Journal
The American journal of tropical medicine and hygiene
Publication Date
Oct 01, 2007
Volume
77
Issue
4
Pages
753–756
Identifiers
PMID: 17978083
Source
Medline
License
Unknown

Abstract

We surveyed 236 potentially pathogenic Acanthamoeba strains, isolated from water sources in the Canary Islands, for the presence of human adenoviruses (HAdV) using a polymerase chain reaction (PCR)-based typing assay. A total of 34 of these strains were found to be positive for adenovirus belonging to four different HAdV serotypes (HAdV-1, 2, 8, and 37). We found that HAdV-2 was the most frequently encountered serotype amongst the Acanthamoeba strains, and their identification was confirmed by a nested PCR specific for this serotype. We showed that Acanthamoeba genotype T4 was highly associated with serotype HAdV-2, whereas Acanthamoeba genotype T3 was most often associated with adenovirus serotypes related to ocular diseases. Based on these data, we suggest that Acanthamoeba should be considered as a potential reservoir and perhaps even a transmitter of adenoviruses to human and other secondary hosts.

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