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Detection of depolymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves.

Authors
Type
Published Article
Journal
Analytical biochemistry
Publication Date
Volume
139
Issue
2
Pages
383–389
Identifiers
PMID: 6433742
Source
Medline
License
Unknown

Abstract

The cup-plate technique makes it possible to detect enzyme activities after diffusion into buffered, substrate-containing agar gels. This technique has been used after nondenaturing blotting transfer in order to detect depolymerizing enzyme activities once analytical protein separation (e.g., by electrophoresis, electrofocusing, or titration curves) has been completed. This rapid and very sensitive method was successfully applied to the enzymes polygalacturonate lyase, polygalacturonate hydrolase, endoglucanase, and xylan hydrolase. Other possible applications are presented.

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