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Detection of Brucella canis infection in dogs by blood culture and bacterial identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Authors
  • Purvis, Tanya J1, 2
  • Krouse, Donna1, 2
  • Miller, Dawn1, 2
  • Livengood, Julia1, 2
  • Thirumalapura, Nagaraja R1, 2
  • Tewari, Deepanker1, 2
  • 1 Kansas State Veterinary Diagnostic Laboratory, Manhattan, KS (Purvis).
  • 2 Pennsylvania Veterinary Laboratory, Pennsylvania Department of Agriculture, Harrisburg, PA (Krouse, Miller, Livengood, Thirumalapura, Tewari).
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
Jul 01, 2017
Volume
29
Issue
4
Pages
586–588
Identifiers
DOI: 10.1177/1040638717704652
PMID: 28381135
Source
Medline
Keywords
License
Unknown

Abstract

Brucella canis was recovered from dogs that were canine brucellosis suspect by blood culture using a modified lysis method. Organism identity was established by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The instrument-provided security library identified the isolates as Brucella species. The isolates were further identified as B. canis with the help of phenotypic and genotypic characteristics. The mass spectral profiles from characterized B. canis isolates, when added to the MALDI-TOF MS standard reference library, allowed successful presumptive identification of B. canis.

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