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Detection of avian influenza virus using an interferometric biosensor.

Authors
  • Xu, Jie
  • Suarez, David
  • Gottfried, David S
Type
Published Article
Journal
Analytical and bioanalytical chemistry
Publication Date
Oct 01, 2007
Volume
389
Issue
4
Pages
1193–1199
Identifiers
PMID: 17710386
Source
Medline
License
Unknown

Abstract

An interferometric biosensor immunoassay for direct and label-less detection of avian influenza through whole virus capture on a planar optical waveguide is described. The assay response is based on index of refraction changes that occur upon binding of virus particles to unique antigen-specific (hemagglutinin) antibodies on the waveguide surface. Three virus subtypes (two H7 and one H8) in buffer solution were tested using both monoclonal and polyclonal capture antibodies. The real-time response of the antigen-antibody interaction was measured and was shown to be concentration-dependent, with detection limits as low as 0.0005 hemagglutination units per milliliter. A simple sandwich assay was shown to further increase the biosensor response.

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