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Detection of Auger enhancement induced in HeLa cells labeled with iododeoxyuridine and irradiated with 150 kV x-rays--Effects of cysteamine and dimethylsulfoxide.

Authors
  • Shinohara, K
  • Nakano, H
  • Ohara, H
Type
Published Article
Journal
Acta oncologica (Stockholm, Sweden)
Publication Date
Jan 01, 1996
Volume
35
Issue
7
Pages
869–875
Identifiers
PMID: 9004765
Source
Medline
License
Unknown

Abstract

We examined the effects of cysteamine and dimethylsulfoxide on the lethality of iododeoxyuridine-labeled HeLa cells irradiated with 150 kV x-rays (effective energy was 48 keV) and compared with those irradiated with 60Co gamma-rays. The protectable fraction was estimated from the regression lines for the reciprocal plots of the concentration of protectors, versus the degree of protection. The protectable fraction by cysteamine was 9.4% lower in iododeoxyuridine-labeled cells than in control cells when they were irradiated with x-rays, whereas no such difference was observed with dimethylsulfoxide. The slopes of the regression lines were steeper (i.e., more difficult to protect) for both protectors in iododeoxyuridine-labeled cells irradiated with x-rays. No such differences were observed in gamma-irradiated cells. The cause for these differences are attributable to Auger enhancement. The results suggest that at least a portion of Auger enhancement is not protectable by cysteamine and is hard to be protected by both protectors cysteamine and dimethylsulfoxide.

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