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Detection of anti-feline infectious peritonitis virus activity of a Chinese herb extract using geneLEAD VIII, a fully automated nucleic acid extraction/quantitative PCR testing system.

Authors
  • Nishijima, Rui1
  • Endo, Takuro2
  • Gankhuyag, Enkhjavkhlan1
  • Khin, Shwe Thiri Maung Maung2
  • Jafar, Sheikhy Mohammad2
  • Shinohara, Yuta3
  • Tanaka, Yoshikazu4
  • Sawakami, Kazumi5
  • Yohda, Masafumi1
  • Furuya, Tetsuya2
  • 1 Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan. , (Japan)
  • 2 Laboratory of Veterinary Infectious Diseases, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan. , (Japan)
  • 3 Iskra Industry Co., Ltd., Tokyo, Japan. , (Japan)
  • 4 Department of Veterinary Hygiene, Veterinary School, Nippon Veterinary and Life Science University, Tokyo, Japan. , (Japan)
  • 5 Precision System Science Co., Ltd., Chiba, Japan. , (Japan)
Type
Published Article
Journal
The Journal of veterinary medical science
Publication Date
Mar 30, 2023
Volume
85
Issue
4
Pages
443–446
Identifiers
DOI: 10.1292/jvms.22-0185
PMID: 36806247
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The geneLEAD VIII is a fully-automated nucleic acid extraction/quantitative PCR equipment developed by Precision System Science Co., Ltd., (PSS). To take advantage of its capability, we developed a quantitative assay system to measure growth of animal viruses. The system was used to assay one of the Chinese herbal extracts whose anti-malarial activities were previously reported and demonstrated its dose-dependent anti-viral activity against feline infectious peritonitis virus (FIPV), a feline coronavirus causing the fatal diseases in cats, and relatively low cell toxicity. The assay developed in this study is useful to screen antiviral drugs and the anti-FIPV activity of the herbal extract identified have a potential to lead to development of new drugs against FIPV and other coronaviruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

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