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Detecting the Dominant T and B Epitopes of Klebsiella pneumoniae Ferric Enterobactin Protein (FepA) and Introducing a Single Epitopic Peptide as Vaccine Candidate

Authors
  • Nemati Zargaran, Fatemeh1
  • Akya, Alisha1
  • Ghadiri, Keyghobad1
  • Ranjbarian, Parivash1
  • Rostamian, Mosayeb1
  • 1 Kermanshah University of Medical Sciences,
Type
Published Article
Journal
International Journal of Peptide Research and Therapeutics
Publisher
Springer-Verlag
Publication Date
Jun 30, 2021
Pages
1–13
Identifiers
DOI: 10.1007/s10989-021-10247-3
PMID: 34226823
PMCID: PMC8243051
Source
PubMed Central
Keywords
Disciplines
  • Article
License
Unknown

Abstract

Klebsiella pneumoniae causes various human infections. Ferric enterobactin protein (FepA) is a conserved protein of K. pneumoniae with high immunogenicity. In the present study, using comprehensive in silico approaches the T and B cell-specific epitopes of K. pneumoniae FepA were identified. The T (both class I and class II) and B (both linear and conformational) epitopes of FepA were predicted using prediction tools. The predicted epitopes were screened for human similarity, immunogenicity, antigenicity, allergenicity, toxicity, conservancy, structural and physicochemical suitability, and in case of T epitopes binding to HLA alleles, using numerous immune-informatics, homology modeling, and molecular docking approaches. These analyses led to introduce the most dominant FepA epitopes that are appropriate for vaccine development. Furthermore, we introduced an antigenic peptide containing both T and B epitopes which comprises suitable structural and physiochemical properties needed for vaccine development and it is conserved in many bacteria. Altogether, here the highly immunogenic T and B epitopes of FepA as well as a final epitopic peptide containing both T and B epitopes were found and introduced for future vaccine development studies. It is suggested that the actual efficiency and efficacy of our final epitopic peptide be investigated by in vitro/in vivo testing. Supplementary Information The online version contains supplementary material available at 10.1007/s10989-021-10247-3.

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