Hepatocytes are the target cells of hepatitis C virus (HCV), but RNA virus has been described in other extrahepatic compartments such as dendritic cells, lymphocytes, macrophages and platelets. Platelets are responsible for carrying the virus in infected patient and, despite, the absence of the main receptor associated with the entry of the virus into the target cells, the CD81, HCV is able to interact with platelets after in vitro incubation. The virus carried by the platelet presents a greater stability when compared to the virus circulating in the plasma; contributes with the course of the chronic Hepatitis C and is able to function as reservoir for the virus. However, there are few studies about the presence of the virus in megakaryocytes, platelet precursor cells in the bone marrow. Then, the present study aimed to evaluate the presence of HCV in megakaryocytes from uninfected donors after an in vitro exposition to HCV. The excess material from bone marrow donation was used for the isolation of megakaryocytes, the megakaryocytes isolated were in vitro incubated with 100.000 IU / ml HCV genotype 1 (39 hours, 5% of CO2 emission, 3 homogenizations per day at the same time). After incubation, the megakaryocytes were submitted by qualitative and quantitative analysis for the presence of the virus. The results demonstrated that HCV may directly interact with megakaryocytes, although this did not occur in all evaluated cases. This result suggests that the virus found in the platelet in the blood circulation may result from the fragmentation of the infected megakaryocyte besides the direct interaction with the platelet. Thus, the platelet could compose a circulating viral reservoir in the hepatic circulation; this could lead to reinfection and release the virus into the hepatocyte. Besides, this event could produce a new infection and result in a hepatocyte-platelet-hepatocyte cycle, which contributes to viral persistence in the infected patient.