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A detailed lipidomic study of human pathogenic fungi Candida auris.

Authors
  • Shahi, Garima1
  • Kumar, Mohit1, 2
  • Kumari, Sonam2
  • Rudramurthy, Shivaprakash M3
  • Chakrabarti, Arunaloke3
  • Gaur, Naseem A2
  • Singh, Ashutosh4
  • Prasad, Rajendra1
  • 1 Amity Institute of Integrative Science and Health and Amity Institute of Biotechnology, Amity University Gurugram,, Haryana, 122413, India. , (India)
  • 2 Yeast Biofuel Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India. , (India)
  • 3 Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research,, Chandigarh, 160012, India. , (India)
  • 4 Department of Biochemistry, University of Lucknow, Lucknow, Uttar Pradesh, 226007, India. , (India)
Type
Published Article
Journal
FEMS Yeast Research
Publisher
Oxford University Press
Publication Date
Sep 01, 2020
Volume
20
Issue
6
Identifiers
DOI: 10.1093/femsyr/foaa045
PMID: 32756963
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The present study is an attempt to determine the lipid composition of Candida auris and to highlight if the changes in lipids can be correlated to high drug resistance encountered in C. auris. For this, the comparative lipidomics landscape between drug-susceptible (CBS10913T) and a resistant hospital isolate (NCCPF_470033) of C. auris was determined by employing high throughput mass spectrometry. All major groups of phosphoglycerides (PGL), sphingolipids, sterols, diacylglycerols (DAG) and triacylglycerols (TAG), were quantitated along with their molecular lipid species. Our analyses highlighted several key changes where the NCCPF_470033 showed an increase in PGL content, specifically phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol, and phosphatidylethanolamine; odd chain containing lipids and accumulation of 16:1-DAG and 16:0-DAG; depletion of 18:1-TAG and 18:0-TAG. The landscape of molecular species displayed a distinct imprint between isolates. For example, the levels of unsaturated PGLs, contributed by both odd and even-chain fatty acyls were higher in resistant NCCPF_470033 isolate, resulting in a higher unsaturation index. Notwithstanding, several commonalities of lipid compositional changes between resistant C. auris and other Candida spp., the study could also identify distinguishable changes in specific lipid species in C. auris. Together, the data highlights the modulation of membrane lipid homeostasis associated with drug-resistant phenotype of C. auris. © FEMS 2020.

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