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A designed peptide chip: protein fingerprinting technology with a dry peptide array and statistical data mining.

Authors
  • Usui, Kenji
  • Tomizaki, Kin-ya
  • Mihara, Hisakazu
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2009
Volume
570
Pages
273–284
Identifiers
DOI: 10.1007/978-1-60327-394-7_13
PMID: 19649599
Source
Medline
License
Unknown

Abstract

There has recently been increased interest in the potential for microarray technologies to study protein networks in a whole cell system within a single experiment. Protein-detecting microarrays are composed of numerous agents immobilized within a tiny area on solid surfaces to capture targeted proteins and to detect interactions in a high-throughput fashion. In this chapter, in order to extend the usability of peptide microarrays, we describe a novel dry peptide microarray format to obtain protein fingerprint (PFP) data sets and a statistical PFP data manipulation technique to quantitatively analyze targeted proteins.

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