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Design and synthesis of activity probes for glycosidases.

Authors
  • Tsai, Charng-Sheng
  • Li, Yaw-Kuen
  • Lo, Lee-Chiang
Type
Published Article
Journal
Organic Letters
Publisher
American Chemical Society
Publication Date
Oct 17, 2002
Volume
4
Issue
21
Pages
3607–3610
Identifiers
PMID: 12375899
Source
Medline
License
Unknown

Abstract

[structure: see text] A new synthetic route was developed for the preparation of activity probe 1 for beta-glucosidase in this study. The key glycosidation step begins with benzyl p-hydroxyphenylacetate. Benzylic functionalization for the construction of the trapping device was achieved at later stages. Probe 1 was shown to be able to label the target enzyme. This cassette-like design offers great flexibility for future alterations. It would allow the synthetic scheme to expand to other glycosidase probes with different linker/reporter combinations.

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