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Design and screening of M13 phage display cDNA libraries.

Authors
Type
Published Article
Journal
Molecules
1420-3049
Publisher
MDPI AG
Publication Date
Volume
16
Issue
2
Pages
1667–1681
Identifiers
DOI: 10.3390/molecules16021667
PMID: 21330956
Source
Medline

Abstract

The last decade has seen a steady increase in screening of cDNA expression product libraries displayed on the surface of filamentous bacteriophage. At the same time, the range of applications extended from the identification of novel allergens over disease markers to protein-protein interaction studies. However, the generation and selection of cDNA phage display libraries is subjected to intrinsic biological limitations due to their complex nature and heterogeneity, as well as technical difficulties regarding protein presentation on the phage surface. Here, we review the latest developments in this field, discuss a number of strategies and improvements anticipated to overcome these challenges making cDNA and open reading frame (ORF) libraries more readily accessible for phage display. Furthermore, future trends combining phage display with next generation sequencing (NGS) will be presented.

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