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Design of a large-scale femtoliter droplet array for single-cell analysis of drug-tolerant and drug-resistant bacteria.

Authors
  • Iino, Ryota1
  • Matsumoto, Yoshimi
  • Nishino, Kunihiko
  • Yamaguchi, Akihito
  • Noji, Hiroyuki
  • 1 Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo Tokyo, Japan ; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency Tokyo, Japan. , (Japan)
Type
Published Article
Journal
Frontiers in Microbiology
Publisher
Frontiers Media SA
Publication Date
Jan 01, 2013
Volume
4
Pages
300–300
Identifiers
DOI: 10.3389/fmicb.2013.00300
PMID: 24109478
Source
Medline
Keywords
License
Unknown

Abstract

Single-cell analysis is a powerful method to assess the heterogeneity among individual cells, enabling the identification of very rare cells with properties that differ from those of the majority. In this Methods Article, we describe the use of a large-scale femtoliter droplet array to enclose, isolate, and analyze individual bacterial cells. As a first example, we describe the single-cell detection of drug-tolerant persisters of Pseudomonas aeruginosa treated with the antibiotic carbenicillin. As a second example, this method was applied to the single-cell evaluation of drug efflux activity, which causes acquired antibiotic resistance of bacteria. The activity of the MexAB-OprM multidrug efflux pump system from Pseudomonas aeruginosa was expressed in Escherichia coli and the effect of an inhibitor D13-9001 were assessed at the single cell level.

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