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Design of electrical stimulation bioreactors for cardiac tissue engineering.

Authors
  • Tandon, N
  • Marsano, A
  • Cannizzaro, C
  • Voldman, J
  • Vunjak-Novakovic, G
Type
Published Article
Journal
Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Publication Date
Jan 01, 2008
Volume
2008
Pages
3594–3597
Identifiers
DOI: 10.1109/IEMBS.2008.4649983
PMID: 19163486
Source
Medline
License
Unknown

Abstract

Electrical stimulation has been shown to improve functional assembly of cardiomyocytes in vitro for cardiac tissue engineering. Carbon electrodes were found in past studies to have the best current injection characteristics. The goal of this study was to develop rational experimental design principles for the electrodes and stimulation regime, in particular electrode configuration, electrode ageing, and stimulation amplitude. Carbon rod electrodes were compared via electrochemical impedance spectroscopy (EIS) and we identified a safety range of 0 to 8 V/cm by comparing excitation thresholds and maximum capture rates for neonatal rat cardiomyocytes cultured with electrical stimulation. We conclude with recommendations for studies involving carbon electrodes for cardiac tissue engineering.

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