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Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains.

Authors
Type
Published Article
Journal
Cell biochemistry and biophysics
Publication Date
Volume
48
Issue
1
Pages
45–53
Identifiers
PMID: 17703067
Source
Medline

Abstract

In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.

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