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Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains.

Authors
Type
Published Article
Journal
Cell biochemistry and biophysics
Publication Date
Volume
48
Issue
1
Pages
45–53
Identifiers
PMID: 17703067
Source
Medline
License
Unknown

Abstract

In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.

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