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Deregulation of immunoglobulin gene transcription in the Hodgkin-Reed Sternberg cell line L1236.

Authors
Type
Published Article
Journal
British journal of haematology
Publication Date
Volume
115
Issue
2
Pages
326–328
Identifiers
PMID: 11703330
Source
Medline
License
Unknown

Abstract

Hodgkin-Reed Sternberg (H-RS) cells harbour clonal immunoglobulin gene (Ig) rearrangements in almost all cases of classical Hodgkin's disease but lack Ig gene expression. In the H-RS cell line L1236, a somatic mutation of the Ig heavy-chain gene promoter octamer motif has been described as a putative reason for absence of Ig gene expression. We addressed transcriptional activity of this mutated promoter by performing reporter gene studies and gel retardation assays. The results showed that the mutation outside the coding region of the rearranged Ig gene in L1236 cells leads to downregulation of Ig gene expression in H-RS cells.

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