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Deoxynivalenol and zearalenone in Fusarium-contaminated wheat in Mexico City.

Authors
Type
Published Article
Journal
Food additives & contaminants. Part B, Surveillance
Publication Date
Volume
4
Issue
1
Pages
71–78
Identifiers
DOI: 10.1080/19393210.2011.551944
PMID: 24779666
Source
Medline
Keywords
  • Fusarium Toxins
  • Cereals
  • Mycotoxins
  • Trichothecenes
  • Zearalenone

Abstract

Fusarium spp. invasion causes head blight, a destructive disease in the world's main wheat-growing areas, and deoxynivalenol (DON) and zearalenone (ZEA) contamination in cereal-based products. No data are available on the relationship between Fusarium spp. on commercial wheat samples in Mexico City and the presence of mycotoxins. A total of 30 wheat samples were subject to a PCR method involving genes of the trichothecene and zearalenone biosynthesis pathways to detect the presence of Fusarium. Detection and quantification of DON and ZEA was performed using liquid chromatography coupled to UV detection. PCR indicated the presence of the Tri5 and PKS4 genes in 16.7 and 23.3% of samples, respectively. DON and ZEA contamination was found in 51.2 and 71.4% of samples, respectively, where a positive amplification was obtained. This work presents up-to-date information on mycotoxin contamination in Mexico, where improved contamination/exposure data and firm control/monitoring measures are needed.

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