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Density-dependent ERK MAPK expression regulates MMP-9 and influences growth

Authors
  • Marchese, Vincent1
  • Juarez, Jazmin1
  • Patel, Priyal1
  • Hutter-Lobo, Dorothy1
  • 1 Monmouth University, Biology Department, 400 Cedar Avenue, West Long Branch, NJ, 07764, USA , West Long Branch (United States)
Type
Published Article
Journal
Molecular and Cellular Biochemistry
Publisher
Springer-Verlag
Publication Date
Jan 28, 2019
Volume
456
Issue
1-2
Pages
115–122
Identifiers
DOI: 10.1007/s11010-019-03496-w
Source
Springer Nature
Keywords
License
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Abstract

Previous work has shown that expression of the extracellular signal-regulated kinase (ERK) is decreased by high density in normal fibroblast cells, and this was correlated with increased expression of mitogen-activated protein kinase phosphatases. Because of these differences in ERK regulation upon contact inhibition, it is likely that other cellular responses may be influenced by the attainment of a contact-inhibited state. Expression of matrix metalloproteinase-9 and cadherin cleavage were both found to be decreased upon reaching high culture density. Inhibition of ERK activity with the MEK inhibitor PD98059 resulted in increased expression of cadherins, while constitutive activation of ERK through the use of expression of an ERK construct with a D319N sevenmaker mutation resulted in decreased expression of cadherins and enhanced colony formation of HT-1080 fibrosarcoma cells. Taken together, these results corroborate a role for the regulation of ERK upon the attainment of a contact-inhibited state with increased expression of cadherins.

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