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Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.

Authors
  • Lennemann, Nicholas J1
  • Coyne, Carolyn B1
  • 1 a Department of Microbiology and Molecular Genetics , University of Pittsburgh , Pittsburgh , PA 15219.
Type
Published Article
Journal
Autophagy
Publisher
Landes Bioscience
Publication Date
Jan 19, 2017
Identifiers
DOI: 10.1080/15548627.2016.1265192
PMID: 28102736
Source
Medline
Keywords
License
Unknown

Abstract

The endoplasmic reticulum (ER) is exploited by a number of diverse viruses during their infectious life cycles. Flaviviruses, including dengue virus (DENV) and Zika virus (ZIKV), utilize the ER as a source of membranes to establish their replication organelles and to facilitate their assembly and eventual maturation along the secretory pathway. In order to maintain normal homeostasis, host cells have evolved highly efficient processes to dynamically regulate the ER, such as through reticulophagy, a selective form of autophagy that leads to ER degradation. Here, we identify the ER-localized reticulophagy receptor FAM134B as a host cell restriction factor for both DENV and ZIKV. We show that RNAi-mediated depletion of FAM134B significantly enhances both DENV and ZIKV replication at an early stage of the viral life cycle. Consistent with its role as an antiviral host factor, we found that several flaviviruses including DENV, ZIKV, and West Nile virus (WNV), utilize their NS3 virally-encoded proteases to directly cleave FAM134B at a single site within its reticulon homology domain (RHD). Mechanistically, we show that NS3-mediated cleavage of FAM134B blocks the formation of ER and viral protein-enriched autophagosomes, suggesting that the cleavage of FAM134B serves to specifically suppress the reticulophagy pathway. These findings thus point to an important role for FAM134B and reticulophagy in the regulation of flavivirus infection and suggest that these viruses specifically target these pathways to promote viral replication.

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