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Demonstration of C1q - binding to collagen - anticollagen immune complexes in synovial fluids of RA-patients.

Authors
  • Menzel, J
  • Steffen, C
Type
Published Article
Journal
Zeitschrift für Rheumatologie
Publication Date
Jan 01, 1982
Volume
41
Issue
2
Pages
47–49
Identifiers
PMID: 6285637
Source
Medline
License
Unknown

Abstract

Collagen-anticollagen immune complexes were demonstrated indirectly in RA synovial fluids by digestion with collagenase and subsequent antibody determination. Elimination of C1q was performed by affinity chromatography using an anti-C1q column. Binding of C1q to collagen-anticollagen complexes was proven by demonstrating that collagenase-caused liberation of collagen antibodies was abolished or distinctly reduced after passage through the anti-C1q column. Binding of C1q to collagen-anticollagen immune complexes was proven via the fixation of these aggregates onto the anti-C1q column. This was achieved in those 7 RA synovial fluids which contained distinctly demonstrable collagen-anticollagen complexes.

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