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Delivery of florfenicol in veterinary medicine through a PLGA-based nanodelivery system: improving its performance and overcoming some of its limitations.

Authors
  • Trif, Emilia1
  • Cerbu, Constantin2
  • Astete, Carlos E3
  • Libi, Sumit3
  • Pall, Emoke1
  • Tripon, Septimiu4, 5
  • Olah, Diana1
  • Potârniche, Adrian Valentin1
  • Witkowski, Lucjan6
  • Brudască, Gheorghe Florinel1
  • Spînu, Marina1
  • Sabliov, Cristina M3
  • 1 Department of Infectious Diseases, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. , (Oman)
  • 2 Department of Infectious Diseases, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. [email protected]. , (Oman)
  • 3 Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA, 70803, USA.
  • 4 Department of Molecular Biology and Biotechnology, Electron Microscopy Laboratory, Biology and Geology Faculty, Babes-Bolyai University, Cluj-Napoca, Romania. , (Oman)
  • 5 Electron Microscopy Integrated Laboratory, National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania. , (Oman)
  • 6 Laboratory of Veterinary Epidemiology and Economic, Institute of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Warsaw, Poland. , (Poland)
Type
Published Article
Journal
Veterinary Research Communications
Publisher
Springer-Verlag
Publication Date
Feb 01, 2024
Volume
48
Issue
1
Pages
259–269
Identifiers
DOI: 10.1007/s11259-023-10205-y
PMID: 37648880
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

As is the case with other veterinary antibiotics, florfenicol (FFC) faces certain limitations, such as low solubility in water, or the fact that it is reported to interfere with the immune response after some immunoprofilactic actions in livestock. Aiming to improve its efficacy and overall performance, FFC was loaded into a polymeric nanobased delivery system by succesfully using the emulsion-evaporation technique. The poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with FFC were characterized in terms of size (101 ± 0.52 nm), zeta potential (26.80 ± 1.30 mV) and poly-dispersity index (0.061 ± 0.019). The achieved loading was 2.24 μg FFC/mg of NPs, with an entrapment efficiency of 7.9%. The antimicrobial effect, the anti-biofilm formation and the cytotoxicity properties of the NPs were evaluated. The results indicated a MIC decreased by ~97.13% for S. aureus, 99.33% for E.coli and 64.1% for P. aeruginosa when compared to free FFC. The minimum inhibitory concentration (MIC) obtained indicated the potential for using a significantly lower dose of florfenicol. The delivery system produced biofilm inhibition while showing no cytotoxic effects when tested on porcine primary fibroblasts and horse mesenchymal stem cells. These findings suggest that florfenicol can be improved and formulations optimized for use in veterinary medicine through its incorporation into a nanobased delivery system designed to release in a controlled manner over time. © 2023. The Author(s), under exclusive licence to Springer Nature B.V.

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