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Degradation of endocrine disrupting chemicals by genetic transformants with two lignin degrading enzymes in Phlebia tremellosa

Authors
  • Kum, Hyunwoo1
  • Lee, Sungsuk2
  • Ryu, Sunhwa2
  • Choi, Hyoung T.1
  • 1 Kangwon National University, Molecular Microbiology Lab, Department of Biochemistry, Chunchon, 200-701, Republic of Korea , Chunchon (South Korea)
  • 2 Korea Forest Research Institute, Division of Forest Bioenergy, Seoul, 130-712, Republic of Korea , Seoul (South Korea)
Type
Published Article
Journal
The Journal of Microbiology
Publisher
The Microbiological Society of Korea
Publication Date
Oct 01, 2011
Volume
49
Issue
5
Pages
824–827
Identifiers
DOI: 10.1007/s12275-011-1230-y
Source
Springer Nature
Keywords
License
Yellow

Abstract

A white rot fungus Phlebia tremellosa produced lignin degrading enzymes, which showed degrading activity against various recalcitrant compounds. However, manganese peroxidase (MnP) activity, one of lignin degrading enzymes, was very low in this fungus under various culture conditions. An expression vector that carried both the laccase and MnP genes was constructed using laccase genomic DNA of P. tremellosa and MnP cDNA from Polyporus brumalis. P. tremellosa was genetically transformed using the expression vector to obtain fungal transformants showing increased laccase and MnP activity. Many transformants showed highly increased laccase and MnP activity at the same time in liquid medium, and three of them were used to degrade endocrine disrupting chemicals. The transformant not only degraded bisphenol A and nonylphenol more rapidly but also removed the estrogenic activities of the chemicals faster than the wild type strain.

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