Affordable Access

deepdyve-link
Publisher Website

Degradation of Bisphenol A by Purified Laccase from Trametes villosa

Authors
  • Fukuda, Tetsuya
  • Uchida, Hiroyuki
  • Takashima, Yoshiko
  • Uwajima, Takayuki
  • Kawabata, Takahiro
  • Suzuki, Motoshi
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publication Date
Jan 01, 2001
Volume
284
Issue
3
Pages
704–706
Identifiers
DOI: 10.1006/bbrc.2001.5021
Source
Elsevier
Keywords
License
Unknown

Abstract

Degradation of bisphenol A (BPA), an endocrine-disrupting chemical, was studied with a purified laccase from the basidiomycete Trametes villosa. SDS–polyacrylamide gel electrophoresis of the purified laccase gave one single band with a mobility corresponding to MW 65 kDa. The absorption spectrum showed the characteristics of a blue copper protein with a maximum peak at 600 nm. HPLC analysis revealed that 2.2 μmol BPA were degraded by incubation with 1.5 units of the purified laccase in a total volume of 1.0 ml at pH 6.0 and 60°C for 3 h. The enzyme reaction proceeded rapidly without requirement of mediators for the electron transfer. Isolation and identification of several reaction products are in progress, in which one product was identified as 4-isopropenylphenol by a gas chromatography–mass spectrophotometer.

Report this publication

Statistics

Seen <100 times