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Decrease in platelet reduced glutathione increases lipoxygenase activity and decreases vitamin E.

Authors
  • Calzada, C1
  • Véricel, E
  • Lagarde, M
  • 1 Institut National de la Santé et de la Recherche Médicale U.205, Chimie Biologique INSA, Villeurbanne, France. , (France)
Type
Published Article
Journal
Lipids
Publication Date
Sep 01, 1991
Volume
26
Issue
9
Pages
696–699
Identifiers
PMID: 1762513
Source
Medline
License
Unknown

Abstract

Unstimulated normal human blood platelets were treated with azodicarboxylic acid bis(dimethylamide) (diamide), a thiol-oxidizing agent. Oxygenated arachidonic acid (AA) metabolites, malondialdehyde (MDA), and tocopherols were then quantified by high-performance liquid chromatography (HPLC). Diamide treatment partially decreased the amount of reduced glutathione (GSH) content and induced a subsequent decrease in peroxidase activity. However, formation of 12-hydroxy-eicosatetraenoic acid (12-HETE), the end-product of lipoxygenation of AA, increased. Formation of MDA, a marker of overall lipid peroxidation, was also enhanced. Furthermore, platelet alpha-tocopherol, but not gamma-tocopherol, significantly decreased. These results indicate that enhanced "basal" lipoxygenase activity, as a marker of specific AA oxygenation, may be linked to decreased platelet antioxidant status.

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