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Structure of the Head of the Bartonella Adhesin BadA

Authors
Journal
PLoS Pathogens
1553-7366
Publisher
Public Library of Science
Publication Date
Volume
4
Issue
8
Identifiers
DOI: 10.1371/journal.ppat.1000119
Keywords
  • Research Article
  • Biochemistry/Membrane Proteins And Energy Transduction
  • Biophysics/Experimental Biophysical Methods
  • Biophysics/Membrane Proteins And Energy Transduction
  • Biophysics/Structural Genomics
  • Computational Biology/Macromolecular Sequence Analysis
  • Computational Biology/Macromolecular Structure Analysis
  • Computational Biology/Protein Homology Detection
  • Computational Biology/Protein Structure Prediction
  • Evolutionary Biology/Microbial Evolution And Genomics
  • Infectious Diseases/Bacterial Infections
  • Microbiology/Medical Microbiology
  • Microbiology/Microbial Evolution And Genomics
Disciplines
  • Biology
  • Ecology
  • Geography
  • Mathematics

Abstract

Trimeric autotransporter adhesins (TAAs) are a major class of proteins by which pathogenic proteobacteria adhere to their hosts. Prominent examples include Yersinia YadA, Haemophilus Hia and Hsf, Moraxella UspA1 and A2, and Neisseria NadA. TAAs also occur in symbiotic and environmental species and presumably represent a general solution to the problem of adhesion in proteobacteria. The general structure of TAAs follows a head-stalk-anchor architecture, where the heads are the primary mediators of attachment and autoagglutination. In the major adhesin of Bartonella henselae, BadA, the head consists of three domains, the N-terminal of which shows strong sequence similarity to the head of Yersinia YadA. The two other domains were not recognizably similar to any protein of known structure. We therefore determined their crystal structure to a resolution of 1.1 Å. Both domains are β-prisms, the N-terminal one formed by interleaved, five-stranded β-meanders parallel to the trimer axis and the C-terminal one by five-stranded β-meanders orthogonal to the axis. Despite the absence of statistically significant sequence similarity, the two domains are structurally similar to domains from Haemophilus Hia, albeit in permuted order. Thus, the BadA head appears to be a chimera of domains seen in two other TAAs, YadA and Hia, highlighting the combinatorial evolutionary strategy taken by pathogens.

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