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Human cytomegalovirus induces a cellular deoxyguanosine kinase, also interacting with Acyclovir

Authors
Journal
FEMS Microbiology Letters
0378-1097
Publisher
Oxford University Press
Publication Date
Keywords
  • Human Cytomegalovirus
  • Deoxyguanosine Kinase
  • Acyclovir
Disciplines
  • Biology

Abstract

Abstract A cytosol deoxyguanosine kinase (dGK) is induced in either growing or human cytomegalovirus (HCMV, AD169)-infected human fibroblasts (HEF). Data obtained from polyacrylamide gel electrophoresis, heat inactivation and phosphorylation kinetic experiments proved that these dGKs are identical, but completely differ from HCMV-induced thymidine kinase (TK) or deoxycytidine kinase (dCK). In contrast to TK or dCK, only dGK interacts with Acyclovir ( K i = 590 μM). It is suggested that dGK is an important enzyme determining the antiviral activity of Acyclovir.

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