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De novo generation of helper virus-satellite chimera RNAs results in disease attenuation and satellite sequence acquisition in a host-dependent manner.

Authors
  • Pyle, Jesse D1
  • Scholthof, Karen-Beth G2
  • 1 Department of Plant Pathology & Microbiology, Texas A&M University, College Station, TX 77843, United States. Electronic address: [email protected] , (United States)
  • 2 Department of Plant Pathology & Microbiology, Texas A&M University, College Station, TX 77843, United States. Electronic address: [email protected] , (United States)
Type
Published Article
Journal
Virology
Publisher
Elsevier
Publication Date
Jan 15, 2018
Volume
514
Pages
182–191
Identifiers
DOI: 10.1016/j.virol.2017.11.006
PMID: 29197268
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Panicum mosaic virus (PMV) is a helper RNA virus for satellite RNAs (satRNAs) and a satellite virus (SPMV). Here, we describe modifications that occur at the 3'-end of a satRNA of PMV, satS. Co-infections of PMV+satS result in attenuation of the disease symptoms induced by PMV alone in Brachypodium distachyon and proso millet. The 375 nt satS acquires ~100-200 nts from the 3'-end of PMV during infection and is associated with decreased abundance of the PMV RNA and capsid protein in millet. PMV-satS chimera RNAs were isolated from native infections of St. Augustinegrass and switchgrass. Phylogenetic analyses revealed that the chimeric RNAs clustered according to the host species from which they were isolated. Additionally, the chimera satRNAs acquired non-viral "linker" sequences in a host-specific manner. These results highlight the dynamic regulation of viral pathogenicity by satellites, and the selective host-dependent, sequence-based pressures for driving satRNA generation and genome compositions. Copyright © 2017 Elsevier Inc. All rights reserved.

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