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De novo chromosome-level assembly of the Centella asiatica genome.

Authors
  • Pootakham, Wirulda1
  • Naktang, Chaiwat2
  • Kongkachana, Wasitthee2
  • Sonthirod, Chutima2
  • Yoocha, Thippawan2
  • Sangsrakru, Duangjai2
  • Jomchai, Nukoon2
  • U-Thoomporn, Sonicha2
  • Romyanon, Kanokwan3
  • Toojinda, Theerayut3
  • Tangphatsornruang, Sithichoke4
  • 1 National Omics Center, National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand. Electronic address: [email protected] , (Thailand)
  • 2 National Omics Center, National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand. , (Thailand)
  • 3 National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand. , (Thailand)
  • 4 National Omics Center, National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand. Electronic address: [email protected] , (Thailand)
Type
Published Article
Journal
Genomics
Publisher
Elsevier
Publication Date
Jul 01, 2021
Volume
113
Issue
4
Pages
2221–2228
Identifiers
DOI: 10.1016/j.ygeno.2021.05.019
PMID: 34022344
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Centella asiatica is a herbaceous, perennial species indigenous to India and Southeast Asia. C. asiatica possesses several medicinal properties: anti-aging, anti-inflammatory, wound healing and memory enhancing. The lack of available genomics resources significantly impedes the improvement of C. asiatica varieties through molecular breeding. Here, we combined the 10× Genomics linked-read technology and the long-range HiC technique to obtain the genome assembly. The final assembly contained nine pseudomolecules, corresponding to the haploid chromosome number in C. asiatica. These nine chromosomes covered 402,536,584 bases or 93.6% of the 430-Mb assembly. Comparative genomics analyses based on single-copy orthologous genes showed that C. asiatica and the common ancestor of Coriandrum sativum (coriander) and Daucus carota (carrot) diverged about 48 million years ago. This assembly provides a valuable reference genome for future molecular studies, varietal development through marker-assisted breeding and comparative genomics studies in C. asiatica. Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.

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