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Differential inactivation of transforming DNAin vitroandin vivoby 4-hydroxyaminoquinoline 1-oxide

Authors
Journal
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
0027-5107
Publisher
Elsevier
Publication Date
Volume
13
Issue
3
Identifiers
DOI: 10.1016/0027-5107(71)90106-0

Abstract

Abstract An Hcr − strain of Bacillus subtilis showed about 10-fold higher sensitivity to killing by 4-hydroxyaminoquinoline 1-oxide (4HAQO) than the parental Hcr + strain, and the same differential sensitivity was observed between the same pair of strains after UV irradiation or 4-nitroquinoline 1-oxide (4NQO) treatment. DNA extracted from Hcr − cells treated with 4HAQO showed higher transforming activity when assayed on Hcr + cells than on Hcr − cells in parallel to the case of DNA extracted from 4NQO-treated cells. However, DNA treated in vitro with 4HAQO in buffer solution showed the same transforming activity on both Hcr + and Hcr − cells. These results indicate that 4HAQO damage induced in DNA in vivo but not in vitro is repairable by the excision-repair mechanism.

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