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Isolation and purification of cortisol-binding immunoglobulin by affinity chromatography

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
58
Issue
2
Identifiers
DOI: 10.1016/s0009-8981(75)80010-6

Abstract

Summary Cortisol-binding IgG is isolated from the serum of immunized rabbits by affinity chromatography on sepharose-coupled antigen. The preparation of this solid phase, the chromatography and the reactions to characterize the isolated immunoglobulin are described. [t ]

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