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The Influence of Dystrophin on Lateral Diffusion of Proteins in Sarcolemma of L-185 and C2 Myoblasts and Mature Striated Muscle Cells of Rats and Mice, as Measured by FRAP Technique

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
215
Issue
1
Identifiers
DOI: 10.1006/bbrc.1995.2434
Disciplines
  • Biology

Abstract

Abstract The expression of dystrophin can he suppressed in cultured skeletal muscle cells (coded L-185 from rat, and C2 from mouse) after a proper genetic manipulation. The influence of presence or absence of dystrophin on the lateral diffusion constant of Con-A-receptors was studied in the cell membrane of such cells (and also of mature skeletal muscle fibres of rat and mouse) by means of the fluorescence recovery after photobleaching (FRAP) technique, applying a novel fluorescent label called Con-A-BODIPY-FI conjugate. It has been established that the normal maturation of myoblasts into skeletal muscle fibres involves a significant decrease of the mobility of Con-A receptors in the sarcolemma. In the absence of dystrophin, this maturation process cannot take place; the membrane proteins display an increasing mobility during the culture time, which is of lethal effect for these cells.

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