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An enzymatic fluorometric assay forl-lysine in plasma and tissue

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
131
Issue
2
Identifiers
DOI: 10.1016/0003-2697(83)90194-x
Keywords
  • Lysine Measurement
  • Amino Acids
  • Fluorescamine
  • L-Lysine Decarboxylase
  • Fluorometry

Abstract

Abstract A simple, specific, and reliable method has been developed for the determination of l-lysine in blood plasma and tissue. The l-lysine in the sample is decarboxylated enzymatically, and fluorescamine is added to a pentan-1-ol extract of the cadaverine formed. This produces a stable product which is measured fluorometrically.

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